[1]高宗军,徐海龙,夏璐.地下水人工回灌含水介质微生物堵塞试验研究[J].水文地质工程地质,2020,47(3):8-16.[doi:10.16030/j.cnki.issn.1000 -3665.201909056]
 GAO Zongjun,XU Hailong,XIA Lu.An experimental study of bioclogging of aquifer media during artificial reinjection of groundwater[J].Hydrogeology & Engineering Geology,2020,47(3):8-16.[doi:10.16030/j.cnki.issn.1000 -3665.201909056]
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地下水人工回灌含水介质微生物堵塞试验研究()
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《水文地质工程地质》[ISSN:1000-3665/CN:11-2202/P]

卷:
47卷
期数:
2020年3期
页码:
8-16
栏目:
水 文 地 质
出版日期:
2020-05-15

文章信息/Info

Title:
An experimental study of bioclogging of aquifer media during artificial reinjection of groundwater
文章编号:
1000 -3665(2020)03 -0008 -09
作者:
高宗军徐海龙夏璐
山东科技大学地球科学与工程学院,山东 青岛266590
Author(s):
GAO Zongjun XU Hailong XIA Lu
College of Earth Science and Engineering, Shandong University of Science and Technology, Qingdao, Shandong266590,China
关键词:
地下水人工回灌微生物堵塞渗透系数高通量测序细菌群落
Keywords:
artificial reinjection of groundwater bioclogging coefficient of permeability high -throughput sequencing bacterial community
分类号:
P641.25
DOI:
10.16030/j.cnki.issn.1000 -3665.201909056
摘要:
地下水人工回灌过程中,含水介质的堵塞除机械颗粒沉积和化学沉淀外,微生物起重要的作用。然而,目前探究回灌堵塞过程中以生物量为主要研究内容反映生物堵塞的发生,对其堵塞发生过程中微生物菌群组成结构的研究较少。采用室内一维渗流试验,开展含水介质微生物堵塞过程研究。分析含水介质渗透性、营养盐指标及胞外聚合物含量时空动态变化,利用16SrRNA高通量测序技术,深入分析微生物群落多样性和结构特征,从分子水平上揭示含水介质回灌堵塞过程中微生物群落特征及其演替规律。研究结果表明,随着回灌持续,含水介质渗透性呈现非线性降低趋势;空间上,堵塞主要发生在介质表层;时间上,各层介质渗透性依次经历稳定波动—快速下降—缓慢下降—趋于稳定4个阶段。含水介质附着细菌主要分布于26个门,65个纲,162个目,258个科,425个属,767个种。随着回灌的进行,细菌群落丰度、多样性及均匀度均呈现降低趋势。含水介质上附着细菌群落结构发生明显演替。菌群以变形菌门为优势菌门,拟杆菌门为次优势菌门;假单胞菌属(Pseudomonas)为主要优势菌属。
Abstract:
In the process of artificial recharge of groundwater, microorganisms play an important role in the blockage of aquifer media besides mechanical particle deposition and chemical precipitation. At present, however, biomass is the main research content to reflect the occurrence of bio -clogging in the process of recharge blockage, and there are few researches on the composition of microbial flora in the process of blockage.One -dimensional seepage test is carried out to examine the microbial blockage process in aquifer media. The temporal and spatial dynamic changes in permeability, index of nutrition salts and extracellular polymeric substances (EPS) of aquifer medium are analyzed.The high -throughput sequencing technique based on 16SrRNA is used to deeply analyze the diversity and structural characteristics of microbial community, and to reveal the characteristics and succession law of microbial community in the process of recharge blockage at the level of molecular. The results show that in the process of recharge, the permeability of aquifer media shows an obvious non -linear decreasing trend. In space, blockage mainly occurs on the surface of medium. In time, the permeability of each layer goes through four stages: stable fluctuation, rapid decrease, slow declines and smooth. The bacteria attached to aquifer media are mainly distributed in 28 phyla, 65 classes, 162 orders, 258 families, 425 genera and 767 species. The abundance, diversity and evenness of bacterial community are decreased. In terms of the structure and composition of flora, proteobacteria is the dominant phylum, followed by actinobacteria, and pseudomonas is the main dominant genus. There is an obvious succession of bacterial community structure attached to the medium.

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备注/Memo

备注/Memo:
收稿日期: 2019 -09 -18; 修订日期: 2019 -11 -19
基金项目: 国家自然科学基金项目(41902250;41641020)
第一作者: 高宗军(1964 -),男,教授,主要从事水文地质研究工作。E -mail:gaozongjun@126.com
更新日期/Last Update: 2020-05-15